DeCIFR — Biodiversity Informatics Pipelines & Visualization
DeCIFR Suite

DeCIFR: Biodiversity Informatics Suite

Center for Integrated Fungal Research (CIFR) · Tools for deciphering the Tree of Life

DeCIFR is a comprehensive suite of biodiversity informatics pipelines and visualization tools to discover, evaluate, and describe taxa of animals, archaea, bacteria, fungi, plants, protists, and viruses at multiple phylogenetic and spatial scales.

Phylogenetic & Network Methods

Tree inference, placement, and network analysis.

T-BAS Toolkit

T-BAS Toolkit

Tree-Based Alignment Selector Toolkit.

Patristic Distance

Patristic Distance

Create patristic distance rings with DendroPy.

MAFFT

MAFFT

Align sequences with MAFFT.

RAxML & FastTree

RAxML & FastTree

Maximum likelihood tree inference.

IQ-TREE

IQ-TREE

Fast maximum likelihood tree inference.

BEAST

BEAST

Bayesian phylogenetic tree inference.

Microsatellite Tree

Microsatellite Tree

Reconstruct tree from microsatellite data.

ASTRAL

ASTRAL

Multispecies coalescent; PhyParts gene-tree conflict.

EPA-ng

EPA-ng

Parallel, highly accurate ML phylogenetic placement.

Graph Inference

Graph Inference

Create networks with TCS or upload graph file.

SplitsTree

SplitsTree

Neighbor-Net splits network inference.

Mesquite Hypha

Mesquite Hypha

Display multiple node support values on trees.

Metabarcoding Methods

Clustering, ASVs, and haplotype tables.

MeShClust

MeShClust

Create haplotype tables.

Cluster Sequences

Cluster Sequences

QIIME (UCLUST) or VSEARCH clustering.

Graph CSV

Graph CSV

Import CSV to graph.

NGMLST

NGMLST

Next-Gen MLST using DADA2.

DADA2

DADA2

Infer exact ASVs from barcodes.

Structure Files

Structure Files

Prepare data for Structure.

Phyloseq

Phyloseq

Create microbiome graphs from phyloseq objects.

Database Search Methods

Taxonomy assignment and external resource queries.

BLAST Taxonomy

BLAST Taxonomy

Select fungal taxonomy using UNITE or NCBI ITS.

Query GenBank

Query GenBank

Download FASTA and metadata.

ITSx

ITSx

Split combined ITS/LSU into separate files.

Create Taxonomy

Create Taxonomy

Create taxonomy CSV from scientific names.

T-BAS Results Database

T-BAS Results Database

View T-BAS results for a run ID.

T-BAS Specimen Database

T-BAS Specimen Database

View specimen-level results.

TreeBASE

TreeBASE

Download NEXUS trees from TreeBASE.

GNPS Library Search

GNPS Library Search

Search GNPS spectral libraries.

GNPS Ontology

GNPS Ontology

Place MASST results on NCBI taxonomy tree.

GenBank IDs

GenBank IDs

Update metadata with genus/species taxids.

File Manipulation & Conversion

Concatenation, conversion, utilities.

Concatenate Loci

Concatenate Loci

Combine loci or create single-locus files.

Merge Vouchers

Merge Vouchers

Merge two voucher tables.

Extend Sequence

Extend Sequence

Extend alignments with additional sequence.

Append Files

Append Files

Append text files into one.

Get Set Results

Get Set Results

Compare lists from two files.

Nwk2nexus

Nwk2nexus

Convert Newick trees to NEXUS.

Sequence Converter

Sequence Converter

Convert sequence files; variable-sites report.

FASTA from List

FASTA from List

Create FASTA using a strain list.

Extract Chromosome FASTA

Extract Chromosome FASTA

Extract chromosome FASTA from map.

Loci from Annotations

Loci from Annotations

Create locus files from phmmer/nhmmer outputs.

Sort FASTA

Sort FASTA

Sort sequences by length.

Rename FASTA headers

Rename FASTA Headers

Rename headers with filename/append.

NGSpeciesID Postprocessing

NGSpeciesID Postprocessing

Process Galaxy run archives.

Population Genetic Methods

Isolation-with-Migration, structure, and more.

Neutrality

Neutrality

Run neutrality tests locally.

IMa3

IMa3

Isolation-with-Migration estimation.

Structure

Structure

Show tree with Structure plots.

IMgc

IMgc

IMgc on CIPRES.

Pharokka

Pharokka

Fast Phage Annotation Tool.

ProphET

ProphET

Prophage Estimation Tool.

Pipelines & Workflow Orchestration

Launch and manage reproducible pipelines.

Galaxy

Galaxy

Tools for population genetics & multi-omics.

Galaxy — PPP

Galaxy — PPP

Popgen Pipeline Platform.

Galaxy — Cassava Virus Evolution

Galaxy — Cassava Virus Evolution

ViralSeq pipeline for cassava mosaic begomoviruses.

Seqera (Nextflow Launchpad)

Seqera

Run and monitor Nextflow pipelines.

Machine Learning

Deep-learning tools for microbiome and phenotype prediction.

PopPhy-CNN

PopPhy-CNN

CNNs to predict host phenotype from metagenomic data.

MiMeNet

MiMeNet

Model microbiome–metabolome relationships using neural nets.

MiMeNet postprocessing

MiMeNet postprocessing

Post-processing & visualization for MiMeNet outputs.

Utilities

Graphs, archives, viewers, and more.

Guide Tree Edit Tool

Guide Tree Edit Tool

Add new accessions to JSON config file for T-BAS guide tree.

Rhs-toxin Extraction Pipeline

Rhs-toxin Extraction Pipeline

Extracts & filters Rhs-toxins from GenBank; summarizes per genome.

PECAT

PECAT

Phased error correction & assembly tool for long reads.

Piechart

Piechart

Create pie charts.

Archive

Archive

Upload archives created by T-BAS/DeCIFR utilities.

Collapse Tree

Collapse Tree

Collapse low-support nodes.

Telescope Viewer

Telescope Viewer

View telescope tree from MEP.

REST

REST

Unzip MEP file for REST.

T-BAS accession

T-BAS accession

Retrieve submitted tree, alignments, and metadata from archive by accession ID.

Merge Metadata

Merge Metadata

Merge columns in T-BAS metadata.

MEP Archive

MEP Archive

Retrieve MEP files from archive by run ID.

Retrieve Names

Retrieve Names

Retrieve names from tree or sequence file.

Update Names

Update Names

Update names in tree or sequence file with replacements from csv.

Extract Taxonomy

Extract Taxonomy

Create taxonomy CSV file from taxon string.

Filter FASTA

Filter FASTA

Create FASTA locus files from genome annotations.

D3 SVG and Canvas

D3 SVG and Canvas

Big Tree Viewer.

How to cite DeCIFR

Carbone, I. & White, J.B. (2025). DeCIFR: a comprehensive suite of biodiversity informatics pipelines and visualization tools. https://doi.org/10.52750/845633