Center for Integrated Fungal Research (CIFR) · Tools for deciphering the Tree of Life
DeCIFR is a comprehensive suite of biodiversity informatics pipelines and visualization tools to discover, evaluate, and describe taxa of animals, archaea, bacteria, fungi, plants, protists, and viruses at multiple phylogenetic and spatial scales.
Tree inference, placement, and network analysis.

Tree-Based Alignment Selector Toolkit.

Create patristic distance rings with DendroPy.

Align sequences with MAFFT.

Maximum likelihood tree inference.

Fast maximum likelihood tree inference.

Bayesian phylogenetic tree inference.

Reconstruct tree from microsatellite data.

Multispecies coalescent; PhyParts gene-tree conflict.

Parallel, highly accurate ML phylogenetic placement.

Create networks with TCS or upload graph file.

Neighbor-Net splits network inference.

Display multiple node support values on trees.
Clustering, ASVs, and haplotype tables.
Taxonomy assignment and external resource queries.

Select fungal taxonomy using UNITE or NCBI ITS.

Download FASTA and metadata.

Split combined ITS/LSU into separate files.

Create taxonomy CSV from scientific names.

View T-BAS results for a run ID.

View specimen-level results.

Download NEXUS trees from TreeBASE.

Search GNPS spectral libraries.

Place MASST results on NCBI taxonomy tree.

Update metadata with genus/species taxids.
Concatenation, conversion, utilities.

Combine loci or create single-locus files.

Merge two voucher tables.

Extend alignments with additional sequence.

Append text files into one.

Compare lists from two files.

Convert Newick trees to NEXUS.

Convert sequence files; variable-sites report.

Create FASTA using a strain list.

Extract chromosome FASTA from map.

Create locus files from phmmer/nhmmer outputs.

Sort sequences by length.

Rename headers with filename/append.

Process Galaxy run archives.
Isolation-with-Migration, structure, and more.
Launch and manage reproducible pipelines.
Deep-learning tools for microbiome and phenotype prediction.
Graphs, archives, viewers, and more.

Add new accessions to JSON config file for T-BAS guide tree.

Extracts & filters Rhs-toxins from GenBank; summarizes per genome.

Phased error correction & assembly tool for long reads.

Create pie charts.

Upload archives created by T-BAS/DeCIFR utilities.

Collapse low-support nodes.

View telescope tree from MEP.

Unzip MEP file for REST.

Retrieve submitted tree, alignments, and metadata from archive by accession ID.

Merge columns in T-BAS metadata.

Retrieve MEP files from archive by run ID.

Retrieve names from tree or sequence file.

Update names in tree or sequence file with replacements from csv.

Create taxonomy CSV file from taxon string.

Create FASTA locus files from genome annotations.

Big Tree Viewer.
Carbone, I. & White, J.B. (2025). DeCIFR: a comprehensive suite of biodiversity informatics pipelines and visualization tools. https://doi.org/10.52750/845633