DeCIFR

Cite as:
Carbone, I & White, J.B. (2025). DeCIFR: a comprehensive suite of biodiversity informatics pipelines and visualization tools. https://doi.org/10.52750/845633.

Phylogenetic and network methods

T-BAS
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T-BAS Toolkit

Tree-Based Alignment Selector Toolkit

patristic distance
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Patristic Distance

Create patristic distance rings with DendroPy

MAFFT tool
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MAFFT

Align sequences with MAFFT

denovo
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RAxML and FastTree

Maximum likelihood tree inference

iqtree
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IQ-TREE

Fast maximum likelihood tree inference

beast
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BEAST

Bayesian phylogenetic tree inference

microsats_tree
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Microsatellite tree

Reconstruct tree from microsatellite data

astral_graphs
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ASTRAL

Multispecies coalescent from gene trees and PhyParts to show gene tree conflict

epang tool
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EPA-ng

Fast, parallel, highly accurate Maximum Likelihood Phylogenetic Placement

tcs graph
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Graph Inference

Create networks using TCS or upload graph file

splitstree
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SplitsTree

Infer splits phylogenetic networks using Neighbor-Net

trees 2 hypha tool
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Mesquite Hypha

Show multiple node support values on tree using Mesquite Hypha


Metabarcoding methods

meshclust
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MeShClust

Create haplotype table with MeShClust

cluster
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Cluster Sequences

Cluster sequences with QIIME (UCLUST) or VSEARCH

csv file to graph
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Graph CSV

Import csv file to graph

dada2 logo
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NGMLST

NGMLST: Next Generation Multilocus Sequence Typing using DADA2

dada2 logo
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DADA2

DADA2: Infer exact amplicon sequence variants (ASVs) from barcode sequences

structure data
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Structure Files

Process data for structure tool

phyloseq
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Phyloseq

Use phyloseq object to create graphs


Database search methods

taxonomy using UNITE ITS
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BLAST Taxonomy

Select fungal taxonomy using UNITE or NCBI ITS database

query genbank
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Query GenBank

Download FASTA and metadata for query of GenBank

Split combined ITS/LSU
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ITSx

Split combined ITS/LSU into separate files with ITSx

query genbank for taxonomy
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Create Taxonomy

Create taxonomy csv file from list of scientific names

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T-BAS Results Database

View T-BAS results from database for a run ID

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T-BAS Specimen Database

View T-BAS results from database for specimens

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Treebase

Download tree NEXUS files from treebase database

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GNPS Library Search

Search in GNPS spectral libraries for metabolite

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GNPS Ontology

Place MASST search results on NCBI taxonomy tree

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GenBank IDs

Update T-BAS metadata with genus, species taxids.


File manipulation and conversion

concat loci
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Concatenate Loci

Concatenate several loci or generate single locus files

merge tables
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Merge Vouchers

Merge two voucher tables into one

extend_sequence
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Extend Sequence

Extend alignment with additional sequence

drap drop
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Append Files

Append several text files into one file

venn diagram
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Get Set Results

Get set results from lists in 2 files

newick to nexus
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Nwk2nexus

Convert Phylogenetic tree from Newick to Nexus format

newick to nexus
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Sequence Converter

Convert sequence file(s) to different format with sites variable report

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FASTA from list

Create new FASTA files using a strain list

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Extract Chromosome FASTA

Extract chromosome FASTA files from a Map file

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Loci from Annotations

Create protein and DNA locus files from annotations using phmmer and nhmmer output, respectively

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Sort FASTA

Sort FASTA file by lengths

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Rename FASTA headers

Rename FASTA headers with filename and append


Population genetic methods

neutrality test
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Neutrality

Install locally and run neutrality test

mep archive
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IMa3

Estimation of migration rates using Isolation-with-Migration (IMa3) models

structure plots
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Structure

Show tree with structure plots

mep archive
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IMgc

IMgc on CIPRES

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Pharokka

Fast Phage Annotation Tool

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ProphET

Prophage Estimation Tool


Galaxy@CIFR

galaxy
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Galaxy

Tools for population genetics and multi‑omics analysis

galaxy
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Galaxy - PPP

Popgen Pipeline Platform (PPP) for population genomic analyses

galaxy
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Galaxy - Cassava Virus Evolution

ViralSeq pipeline for NGS analysis of cassava mosaic begomoviruses


Utilities

pie chart
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Piechart

Create pie charts

archive
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Archive

Upload archive created by TBAS or DeCIFR utility

tree with collapse nodes
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Collapse Tree

Collapse low support nodes on tree

telescope tree
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Telescope Viewer

View telescope tree from MEP file

mep archive
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MEP Archive

Retrieve MEP files from archive by run ID

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Retrieve Names

Retrieve names from tree or sequence file

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Update Names

Update names in tree or sequence file with replacements from csv

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Extract Taxonomy

Create taxonomy CSV file from taxon string

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Filter FASTA

Create FASTA locus files from genome annotations

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CIPRES Jobs

Get CIPRES XML from jobs URL

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CIPRES Jobs Database

Show jobs submitted to CIPRES (admin)

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CIPRES Jobs Database User

Show jobs submitted to CIPRES (user)

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Guide Tree Edit Tool

Add new accessions to JSON config file for T-BAS guide tree

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D3 SVG and Canvas

Big Tree Viewer

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REST

Unzip MEP file for REST

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T-BAS accession

Retrieve submitted tree, alignments, and metadata from archive by accession ID

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Merge Metadata

Merge columns in T-BAS metadata

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PopPhy-CNN

Convolutional neural networks to predict host phenotype from metagenomic data

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Rhs-toxin Extraction Pipeline

This pipeline extracts and filters Rhs-toxins from GenBank files, creating a summary of Rhs sequences for each genome.

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MiMeNet

Exploring microbiome-metabolome relationships using neural networks

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MiMeNet postprocessing

Exploring microbiome-metabolome relationships using neural networks


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